down-regulation of mir-135b in colon adenocarcinoma induced by a tgf-β receptor i kinase inhibitor (sd-208)

نویسندگان

abolfazl akbari colorectal research center, rasoul-akram hospital, iran university of medical sciences, tehran, iran

mohammad hossein ghahremani department of pharmacology and toxicology, faculty of pharmacy, tehran university of medical sciences, tehran, iran

gholam reza mobini cellular and molecular research center, shahrekord university of medical sciences, shahrekord, iran

mahdi abastabar invasive fungi research center, department of medical mycology and parasitology, school of medicine, mazandaran university of medical sciences, sari, iran

چکیده

objective(s):transforming growth factor-β(tgf-β) is involved in colorectal cancer (crc). the sd-208 acts as an anti-cancer agent in different malignancies via tgf-β signaling. this work aims to show the effect of manipulation of tgf-β signaling on some mirnas implicated in crc. materials and methods: we investigated the effects of sd-208 on sw-48, a colon adenocarcinoma cell line. the cell line was treated with 0.5, 1 and 2 μm concentrations of sd-208. then, the xenograft model of colon cancer was established by subcutaneous inoculation of sw-48 cell line into the nude mice. the animals were treated with sd-208 for three weeks. a quantitative real-time pcr was carried out for expression level analysis of selected oncogenic (mir-21, 31, 20a and 135b) and suppressor-mirnas (let7-g, mir-133b, 145 and 200c). data were analyzed using the 2-∆∆ct method through student’s t-test via the graphpad prism software. results: our results revealed that sd-208 could significantly down-regulate the expression of one key onco-mirna, mir-135b, in either sw-48 colon cells (p=0.006) or tumors orthotopically implanted in nude mice (p=0.018). our in silico study also predicted that sd-208 could modulate the expression of potential downstream tumor suppressor targets of the mir135b. conclusion: our data provide novel evidence that anticancer effects of sd-208 (and likely other tgf-β inhibitors) may be owing to their ability to regulate mirnas expression.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Down-regulation of miR-135b in colon adenocarcinoma induced by a TGF-β receptor I kinase inhibitor (SD-208)

  Objective(s):Transforming growth factor-β(TGF-β) is involved in colorectal cancer (CRC). The SD-208 acts as an anti-cancer agent in different malignancies via TGF-β signaling. This work aims to show the effect of manipulation of TGF-β signaling on some miRNAs implicated in CRC. Materials and Methods: We investigated the effects of SD-208 on SW-48, a colon adenocarcinoma cell line. The cell li...

متن کامل

Down-regulation of miR-135b in colon adenocarcinoma induced by a TGF-β receptor I kinase inhibitor (SD-208)

OBJECTIVES Transforming growth factor-β (TGF-β) is involved in colorectal cancer (CRC). The SD-208 acts as an anti-cancer agent in different malignancies via TGF-β signaling. This work aims to show the effect of manipulation of TGF-β signaling on some miRNAs implicated in CRC. MATERIALS AND METHODS We investigated the effects of SD-208 on SW-48, a colon adenocarcinoma cell line. The cell line...

متن کامل

evaluation of sd-208, a tgf-β-ri kinase inhibitor, as an anticancer agent in retinoblastoma

retinoblastoma is the most common intraocular tumor in children resulting from genetic alterations and transformation of mature retinal cells. the objective of this study was to investigate the effects of sd-208, tgf-β-ri kinase inhibitor, on the expression of some mirnas including a mir-17/92 cluster in retinoblastoma cells. prior to initiate this work, the cell proliferation was studied by me...

متن کامل

Evaluation of SD-208, a TGF-β-RI Kinase Inhibitor, as an Anticancer Agent in Retinoblastoma.

Retinoblastoma is the most common intraocular tumor in children resulting from genetic alterations and transformation of mature retinal cells. The objective of this study was to investigate the effects of SD-208, TGF-β-RI kinase inhibitor, on the expression of some miRNAs including a miR-17/92 cluster in retinoblastoma cells. Prior to initiate this work, the cell proliferation was studied by Me...

متن کامل

The Effect of SB431542, TGF-β Receptor Inhibitor, on HCV Replication in PBMCs of Patients with Chronic Hepatitis

Background and Aims: TGF-β is an effective cytokine in the viral replication cycle, which is also highly relevant to the pathogenesis of some viral infections. TGF-β induction by viral proteins is one of the ways to escape the virus from the immune system by inhibiting interferon signaling and other immune system factors.  In recent years, the role of TGF-β and its inhibitor...

متن کامل

MiR-135b is a direct PAX6 target and specifies human neuroectoderm by inhibiting TGF-β/BMP signaling.

Several transcription factors (TFs) have been implicated in neuroectoderm (NE) development, and recently, the TF PAX6 was shown to be critical for human NE specification. However, microRNA networks regulating human NE development have been poorly documented. We hypothesized that microRNAs activated by PAX6 should promote NE development. Using a genomics approach, we identified PAX6 binding site...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
iranian journal of basic medical sciences

جلد ۱۸، شماره ۹، صفحات ۸۵۶-۸۶۱

کلمات کلیدی
[ ' c o l o n c a n c e r ' , ' o n c o g e n i c a n d s u p p r e s s o r m i c r o r n a s ( m i r n a s ) ' , ' s d ' , 2 0 8 , ' t g f ' , ' β r e c e p t o r 1 ( t g β r i ) k i n a s e i n h i b i t o r ' ]

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023